MAN1A1 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86094-1-RR (same clone as 86094-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86094-1-RR (MAN1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86094-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02326-1

  Sandwich ELISA standard curve of MP02326-1, Human MAN1A1 Recombinant Matched Antibody Pair - PBS only. 86094-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20060. 86094-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP02326-1

  Urine of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 0.7 ng/mL with a range of ND-2.2 ng/mL.

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  Sample test of MP02326-1

  Serum of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 11.9 ng/mL with a range of 6.8-24.2 ng/mL.

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  IHC staining of human ovary cancer using 86094-1-RR (same clone as 86094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86094-1-RR (MAN1A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86094-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86094-1-RR (same clone as 86094-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (86094-1-RR, Clone: 250444H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86094-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86094-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86094-1-RR against Human MAN1A1 were performed. The affinity constant is 1.04 nM.

MAN1A1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86094-1-PBS

Clone No.250444H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

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Product Information

86094-1-PBS targets MAN1A1 as part of a matched antibody pair:

MP02326-1: 86094-1-PBS capture and 86094-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20060

  Product name: Recombinant human MAN1A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 72-367 aa of BC065827

  Sequence: VLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEEIEARKGQANCPGSSSSCEVEIQMMKHAWNNYKGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKRWMCNIPRAMEATI

• Full Name: mannosidase, alpha, class 1A, member 1
• Calculated Molecular Weight: 653 aa, 73 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC065827
• Gene Symbol: MAN1A1
• Gene ID (NCBI): 4121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P33908
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).