Biotin-conjugated MAN1A1 Polyclonal antibody

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  IHC staining of human ovary tumor using Biotin-23382

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-23382 (MAN1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using Biotin-23382

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-23382 (MAN1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAN1A1 Polyclonal Antibody for IHC, ELISA

Cat No. Biotin-23382

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

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Tested Applications

• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-23382 targets MAN1A1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAN1A1 fusion protein Ag20060
• Full Name: mannosidase, alpha, class 1A, member 1
• Calculated Molecular Weight: 653 aa, 73 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC065827
• Gene Symbol: MAN1A1
• Gene ID (NCBI): 4121
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33908
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).