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MAN1A2 Polyclonal antibody, PBS Only (14049-1-PBS)

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WB analysis of human placenta using 14049-1-AP (same clone as 14049-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 14049-1-AP (MAN1A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IP experiment of human placenta using 14049-1-AP (same clone as 14049-1-PBS)
IP result of anti-MAN1A2 (IP:14049-1-AP, 4ug; Detection:14049-1-AP 1:200) with human placenta tissue lysate 4000ug. This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14049-1-AP (same clone as 14049-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14049-1-PBS in a different storage buffer formulation.

MAN1A2 Polyclonal antibody, PBS Only

Name: MAN1A2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14049-1-PBS

MAN1A2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14049-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14049-1-PBS targets MAN1A2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5240 Product name: Recombinant human MAN1A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 292-641 aa of BC063300 Sequence: VQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR Predict reactive species
Full Name	mannosidase, alpha, class 1A, member 2
Calculated Molecular Weight	641 aa, 73 kDa
Observed Molecular Weight	73 kDa
GenBank Accession Number	BC063300
Gene Symbol	MAN1A2
Gene ID (NCBI)	10905
RRID	AB_2266033
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60476
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).