Tested Applications
| Positive WB detected in | HEK-293 cells, HCT 116 cells, HepG2 cells, LNCaP cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15243-1-AP targets MAN1B1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7455 Product name: Recombinant human MAN1B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-699 aa of BC002953 Sequence: LFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA Predict reactive species |
| Full Name | mannosidase, alpha, class 1B, member 1 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC002953 |
| Gene Symbol | MAN1B1 |
| Gene ID (NCBI) | 11253 |
| RRID | AB_3669201 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKM7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAN1B1 antibody 15243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAN1B1 Polyclonal antibody (Cat# 15243-1-AP) has 3 total citations. These appear in Molecular Cell, Toxicology and Applied Pharmacology, and Biochemical Pharmacology. The research fields span ER-associated degradation and glycan biology, breast cancer chemoprevention via angiogenesis inhibition, and metastasis prevention through small extracellular vesicle proteomics.
| Species | Application | Title |
|---|---|---|
Mol Cell Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation. | ||
Toxicol Appl Pharmacol Nab-paclitaxel inhibits angiogenesis via the CYR61/integrin α(v)β(3) Axis: Exosomal proteomics insights into breast cancer chemoprevention. | ||
Biochem Pharmacol Apatinib attenuates CRKII-Mediated angiogenesis: Possible implications for metastasis prevention from small extracellular vesicle proteomics. |
