MAN2A1 Polyclonal antibody

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  IHC staining of human stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of mouse stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse stomach using 21132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 40x lens).

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  IF Staining of NIH/3T3 using 21132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MAN2A1 antibody (21132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MAN2A1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 21132-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Alpha-mannosidase 2, AMan II, EC:3.2.1.114, Golgi alpha-mannosidase II, MANA2

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Tested Applications

• Positive IHC detected in: human stomach tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21132-1-AP targets MAN2A1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15536

  Product name: Recombinant human MAN2A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 585-803 aa of BC142656

  Sequence: GTRPFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLEMDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKEDGKHHEVNVQFSWYGTTIKRD

• Full Name: mannosidase, alpha, class 2A, member 1
• Calculated Molecular Weight: 1144 aa, 131 kDa
• GenBank Accession Number: BC142656
• Gene Symbol: MAN2A1
• Gene ID (NCBI): 4124
• RRID: AB_2878816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16706
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-01-2020)

This antibody labels clean doublet bands around the expected molecular weight.

• Applications: Western Blot