Tested Applications
| Positive IHC detected in | human stomach tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21132-1-AP targets MAN2A1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15536 Product name: Recombinant human MAN2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 585-803 aa of BC142656 Sequence: GTRPFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLEMDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKEDGKHHEVNVQFSWYGTTIKRD Predict reactive species |
| Full Name | mannosidase, alpha, class 2A, member 1 |
| Calculated Molecular Weight | 1144 aa, 131 kDa |
| GenBank Accession Number | BC142656 |
| Gene Symbol | MAN2A1 |
| Gene ID (NCBI) | 4124 |
| RRID | AB_2878816 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16706 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAN2A1 antibody 21132-1-AP | Download protocol |
| IHC protocol for MAN2A1 antibody 21132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The customer used it for Western Blot and reported that it labels clean doublet bands at the expected molecular weight, indicating good specificity and performance for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (09-01-2020) | This antibody labels clean doublet bands around the expected molecular weight.
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