MAOB Recombinant monoclonal antibody, PBS Only

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  WB analysis of MCF-7 using 86695-1-RR (same clone as 86695-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 86695-1-RR (MAOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86695-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86695-1-RR (same clone as 86695-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86695-1-RR (MAOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86695-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86695-1-RR (same clone as 86695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86695-1-RR (MAOB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 86695-1-RR (same clone as 86695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86695-1-RR (MAOB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86695-1-PBS in a different storage buffer formulation.

MAOB Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86695-1-PBS

Clone No.251600B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Amine oxidase [flavin-containing] B, EC:1.4.3.21, MAO B, MAO-B, monoamine oxidase B

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Product Information

86695-1-PBS targets MAOB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3253

  Product name: Recombinant human MAOB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC022494

  Sequence: MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDHVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIQSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDT

• Full Name: monoamine oxidase B
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022494
• Gene Symbol: MAOB
• Gene ID (NCBI): 4129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27338
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).