MAOB Recombinant monoclonal antibody

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  WB analysis of MCF-7 using 86695-1-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 86695-1-RR (MAOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86695-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86695-1-RR (MAOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 86695-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86695-1-RR (MAOB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86695-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86695-1-RR (MAOB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAOB Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86695-1-RR

Clone No.251600B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Amine oxidase [flavin-containing] B, EC:1.4.3.21, MAO B, MAO-B, monoamine oxidase B

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86695-1-RR targets MAOB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3253

  Product name: Recombinant human MAOB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC022494

  Sequence: MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDHVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIQSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDT

• Full Name: monoamine oxidase B
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022494
• Gene Symbol: MAOB
• Gene ID (NCBI): 4129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27338
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).