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LC3C-Specific Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 18726-1-AP

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Con: 35 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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LC3C-Specific Antibody WB Recombinant protein protein 18726-1-AP

WB results of LC3C-specific antibody with Recombinant protein LC3A, LC3B and LC3C, by Dr. Christos Zois.

LC3C-Specific Antibody IHC human breast cancer tissue 18726-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18726-1-AP( LC3C-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
Recombinant protein
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
microtubule-associated protein 1 light chain 3 gamma
Calculated molecular weight:
17 kDa
Observed molecular weight:
GenBank accession number:
NM_001004343
Gene ID (NCBI):
440738
Gene symbol
MAP1LC3C
Synonyms
LC3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C

Background

LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.


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