MAP2K4 Polyclonal antibody

MAP2K4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51142-1-AP

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

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Validation Data Gallery

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  WB analysis of mouse brain using 51142-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 51142-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 51142-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51142-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51142-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 4
• Calculated Molecular Weight: 399 aa, 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC036032
• Gene Symbol: MAP2K4
• Gene ID (NCBI): 6416
• RRID: AB_2881252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.