MAP3K1 Polyclonal antibody, PBS Only

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• MAP3K1 Polyclonal antibody, PBS Only (19970-1-PBS)

• 

  WB analysis using 19970-1-AP (same clone as 19970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19970-1-AP (same clone as 19970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 19970-1-AP (same clone as 19970-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 19970-1-AP (same clone as 19970-1-PBS)

  IP result of anti-MAP3K1 (IP:19970-1-AP, 4ug; Detection:19970-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug. This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19970-1-AP (same clone as 19970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 19970-1-AP (same clone as 19970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19970-1-AP (same clone as 19970-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19970-1-AP (MAP3K1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19970-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MAP3K1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 19970-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

19970-1-PBS targets MAP3K1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 1
• Calculated Molecular Weight: 164 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: NM_005921
• Gene Symbol: MAP3K1
• Gene ID (NCBI): 4214
• RRID: AB_2878633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13233
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1.