MAP4K4 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAP4K4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 55247-1-AP

Synonyms

FLH21957, HGK, HPK/GCK like kinase HGK, KIAA0687, MAP4K4, MEK kinase kinase 4, MEKKK 4, Nck interacting kinase, NIK

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Validation Data Gallery

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  WB analysis of Jurkat using 55247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55247-1-AP

  WB result of MAP4K4 antibody (55247-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP4K4 transfected Jurkat cells.

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  WB analysis of PC-3 using 55247-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55247-1-AP

  IP result of anti-MAP4K4 (IP:55247-1-AP, 4ug; Detection:55247-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human skeletal muscle tissue, human placenta tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase kinase kinase 4
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 130-160 kDa
• GenBank Accession Number: NM_004834
• Gene Symbol: MAP4K4
• Gene ID (NCBI): 9448
• RRID: AB_10836939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway; Authors - Lulu Chen
human	WB	Anal Chem; Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro.; Authors - Weili Miao
human	WB	EBioMedicine; Targeting miR-30d reverses pathological cardiac hypertrophy.; Authors - Jin Li
mouse	WB	J Proteome Res; Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.; Authors - Jingzi Zhang
mouse	WB,IF	Neural Regen Res; MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.; Authors - Zheng Zou ; KD Validated
human	IHC	PeerJ; Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.; Authors - Wanzhen Li