MAP4K4 Polyclonal antibody

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• MAP4K4 Polyclonal antibody (55247-1-AP)

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• KD/KO Validated

MAP4K4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 55247-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

EC:2.7.11.1, FLH21957, HGK, HPK/GCK like kinase HGK, HPK/GCK-like kinase HGK

• 

  WB analysis of Jurkat using 55247-1-AP

  WB result of MAP4K4 antibody (55247-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP4K4 transfected Jurkat cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 55247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 55247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 55247-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 55247-1-AP

  IP result of anti-MAP4K4 (IP:55247-1-AP, 4ug; Detection:55247-1-AP 1:300) with Jurkat cells lysate 4000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55247-1-AP (MAP4K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HK-2 cells, Jurkat cells, PC-3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human skeletal muscle tissue, human liver tissue, human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase kinase 4
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 130-160 kDa
• GenBank Accession Number: NM_004834
• Gene Symbol: MAP4K4
• Gene ID (NCBI): 9448
• RRID: AB_10836939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95819
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Cancer Cell; Cancer stem cells orchestrate immune evasion through extracellular vesicle-mediated non-canonical signaling pathways.; Authors - Guangjian Fan
mouse	WB	ACS Nano; Nanoparticle-Patch System for Localized, Effective, and Sustained miRNA Administration into Infarcted Myocardium to Alleviate Myocardial Ischemia-Reperfusion Injury; Authors - Xuerui Chen
human	WB,IHC,CoIP	Adv Sci (Weinh); Peptide Transporter 1-Mediated Dipeptide Transport Promotes Hepatocellular Carcinoma Metastasis by Activating MAP4K4/G3BP2 Signaling Axis; Authors - Feifeng Song
human	WB	Int J Biol Sci; N6-methyladenosine Reader IGF2BP2-modified HMMR Promotes Non-small Cell Lung Cancer Metastasis via Interaction with MAP4K4; Authors - Jiansheng Zhang
rat	WB,IF	J Neuroinflammation; Transcriptome profiling of microRNAs reveals potential mechanisms of manual therapy alleviating neuropathic pain through microRNA-547-3p-mediated Map4k4/NF-κb signaling pathway; Authors - Chongjie Yao
mouse	WB	Phytomedicine; Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway; Authors - Lulu Chen