MAP4K4 Recombinant monoclonal antibody

• 

  WB analysis using 85387-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85387-2-RR (MAP4K4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

MAP4K4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85387-2-RR

Clone No.242721G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.7.11.1, FLH21957, HGK, HPK/GCK like kinase HGK, HPK/GCK-like kinase HGK

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85387-2-RR targets MAP4K4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase kinase 4
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 130-160 kDa
• GenBank Accession Number: NM_004834
• Gene Symbol: MAP4K4
• Gene ID (NCBI): 9448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95819
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway.