MAP7 Polyclonal antibody

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• MAP7 Polyclonal antibody (13446-1-AP)

MAP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13446-1-AP Copied!

Publications (7)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Microtubule-associated protein 7, MAP-7, Epithelial microtubule-associated protein of 115 kDa, Ensconsin, E-MAP-115

• 

  WB analysis of A549 using 13446-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 13446-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 13446-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13446-1-AP (MAP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 13446-1-AP

  IP result of anti-MAP7 (IP:13446-1-AP, 4ug; Detection:13446-1-AP 1:500) with HeLa cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 13446-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13446-1-AP (MAP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 13446-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP7 antibody (13446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 13446-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 13446-1-AP

  Immunofluorescent analysis of Hela cells, using MAP7 antibody 13446-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13446-1-AP targets MAP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4276

  Product name: Recombinant human MAP7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 400-749 aa of BC025777

  Sequence: GRAPLVKVEEATVEERTPAEPEVGPAAPAMAPAPASAPAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREKEERERREQEELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREWEEAERAQRQKEEEARVREEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSRLDVTNSESPEIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI

• Full Name: microtubule-associated protein 7
• Calculated Molecular Weight: 749 aa, 84 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC025777
• Gene Symbol: MAP7
• Gene ID (NCBI): 9053
• RRID: AB_10644485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14244
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The MAP7 antibody (catalog #13446-1-AP) has 7 citations published in Advanced Science, Journal of Medicinal Chemistry, Life Sciences, Frontiers in Pharmacology, BMC Biology, European Journal of Medical Research, and Molecular Biology of the Cell. Associated research fields include nasopharyngeal carcinoma, colorectal cancer, preeclampsia, osteosarcoma, lung adenocarcinoma, microtubule/spindle biology, and neuronal motility.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); N^6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation; Authors - Yin Zhao
		J Med Chem; Discovery of the First Highly Potent and Selective Peptide Inhibitor Targeting Microtubule-MAP7 Interaction via Structure-Based Design for Suppressing Colorectal Cancer Cell Proliferation.; Authors - Miao-Miao Niu
		Life Sci; Cold stress exacerbates preeclampsia pathogenesis by upregulating Map7 expression in placental macrophages.; Authors - Shasha Fan
human	WB	Front Pharmacol; A telomere-related signature for predicting prognosis and assessing immune microenvironment in osteosarcoma; Authors - Shihao Li
human	IF	BMC Biol; Two-step interphase microtubule disassembly aids spindle morphogenesis.; Authors - Nunu Mchedlishvili
human	IP	Eur J Med Res; FBXW7 regulates MAP7 through ubiquitination to affect the phenotypic characteristics and paclitaxel sensitivity of paclitaxel-resistant lung adenocarcinoma cells.; Authors - Yi Zhang ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is a 5-star rating from a Western Blot experiment. The reviewer used a 1/1000 dilution on platelets and confirmed that MAP7 is detected in mouse platelets, indicating the antibody performs well for its intended target.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Toscane (Verified Customer) (02-05-2025)

MAP7 is detected in mouse platelets

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Platelets