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SIN1/MAPKAP1 Polyclonal antibody (15463-1-AP)

SIN1/MAPKAP1 Polyclonal antibody

SIN1/MAPKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15463-1-AP

Publications(14)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, IP-MS, ELISA

Synonyms

MAPKAP1, MIP1, Mitogen-activated protein kinase 2-associated protein 1, SAPK interacting protein 1, SIN1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Freight/Packing: -

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse kidney using 15463-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 15463-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse kidney using 15463-1-AP
IP result of anti-SIN1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 15463-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 15463-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 15463-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15463-1-AP (SIN1 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15463-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SIN1 antibody (15463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (14)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HeLa cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	mouse kidney tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15463-1-AP targets SIN1/MAPKAP1 in WB, IHC, IF/ICC, IP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7753 Product name: Recombinant human MAPKAP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-324 aa of BC002326 Sequence: MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGSRAD Predict reactive species
Full Name	mitogen-activated protein kinase associated protein 1
Calculated Molecular Weight	59 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC002326
Gene Symbol	MAPKAP1
Gene ID (NCBI)	79109
RRID	AB_10598466
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BPZ7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitogen-activated protein kinase 2-associated protein 1 (MAPKAP1) is also named as MIP1 and SIN1. SIN1/MAPKAP1 is a major partner of mTORC2, acting as a scaffold and responsible for the substrate specificity of the mTOR catalytic subunit (PMID: 37877351). Besides as a component of MTORC2, MAPKAP1 also functions as MEKK2 and Ras-interacting protein and involves in regulating MEKK2 activation and RAS signaling (PMID: 31773361).

Protocols

Product Specific Protocols
IF protocol for SIN1/MAPKAP1 antibody 15463-1-AP	Download protocol
IHC protocol for SIN1/MAPKAP1 antibody 15463-1-AP	Download protocol
IP protocol for SIN1/MAPKAP1 antibody 15463-1-AP	Download protocol
WB protocol for SIN1/MAPKAP1 antibody 15463-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	IF	Cell Rep Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways Authors - Tatsuki Yasuda View Article
human	WB	EMBO Rep Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling Authors - Misha Kalarikkal KD Validated View Article
		J Neurooncol SIN1 facilitates glioma progression and is associated with the KRAS/ERK pathway Authors - Haowei Cao View Article
mouse	WB	bioRxiv Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter Authors - Marco Di Gioia View Article
mouse	WB	Cell Rep Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity. Authors - Fangfang Sun View Article
mouse	IF	bioRxiv Ibudilast Protects Retinal Bipolar Cells from Excitotoxic Retinal Damage and Activates the mTOR Pathway Authors - Sumaya Hamadmad View Article

Reviews

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2 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Shile (Verified Customer) (07-24-2025)	The SIN1 rabbit polyclonal antibody works well in our WB (1:500). Applications: Western Blot Primary Antibody Dilution: WB 1:500 Cell Tissue Type: Human Rh30 cancer cell lysates
Shile (Verified Customer) (01-31-2025)	15463-1-AP SIN1 Rabbit PolyAb was working well in our Western blotting (1:500). Applications: Western Blot Primary Antibody Dilution: 1:500 for WB Cell Tissue Type: 293T cell lysates SIN1/MAPKAP1 Antibody Western Blot validation (1:500 for WB dilution) in 293T cell lysates (Cat no:15463-1-AP)