Tested Applications
| Positive WB detected in | mouse kidney tissue, HeLa cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15463-1-AP targets SIN1/MAPKAP1 in WB, IHC, IF/ICC, IP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7753 Product name: Recombinant human MAPKAP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-324 aa of BC002326 Sequence: MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGSRAD Predict reactive species |
| Full Name | mitogen-activated protein kinase associated protein 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC002326 |
| Gene Symbol | MAPKAP1 |
| Gene ID (NCBI) | 79109 |
| RRID | AB_10598466 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BPZ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitogen-activated protein kinase 2-associated protein 1 (MAPKAP1) is also named as MIP1 and SIN1. SIN1/MAPKAP1 is a major partner of mTORC2, acting as a scaffold and responsible for the substrate specificity of the mTOR catalytic subunit (PMID: 37877351). Besides as a component of MTORC2, MAPKAP1 also functions as MEKK2 and Ras-interacting protein and involves in regulating MEKK2 activation and RAS signaling (PMID: 31773361).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SIN1/MAPKAP1 antibody 15463-1-AP | Download protocol |
| IHC protocol for SIN1/MAPKAP1 antibody 15463-1-AP | Download protocol |
| IP protocol for SIN1/MAPKAP1 antibody 15463-1-AP | Download protocol |
| WB protocol for SIN1/MAPKAP1 antibody 15463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SIN1 antibody (Cat# 15463-1-AP) has 14 citations across journals including Nature Biotechnology, Immunity, Molecular Cell, Cell Death & Disease, Cell Reports, EMBO Reports, Molecular Cancer Therapeutics, Glia, Cell Signaling, Journal of Neuro-Oncology, and bioRxiv. Research fields span mTORC2 signaling, oncogenic Ras, epigenetics, pyroptosis, hepatic metabolism, mitochondrial dynamics, retinal neuroprotection, EMT in lung cancer, glioma progression, and host-microbe immunity. Applications include WB, IF, IHC, and IP-MS.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution | ||
Immunity Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infections. | ||
Cell Death Dis Phosphorylation of USP33 by CDK1 stabilizes the mTORC2 component SIN1.
| ||
Cell Death Dis CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer | ||
Reviews
What customers say
Both reviewers (the same user) gave this SIN1 rabbit polyclonal antibody a perfect 5-star rating for Western Blot. They reported consistent performance at a 1:500 dilution in human Rh30 cancer cell lysates and 293T cell lysates, describing it as working well in their WB experiments. Overall, user feedback is highly positive with no reported issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shile (Verified Customer) (07-24-2025) | The SIN1 rabbit polyclonal antibody works well in our WB (1:500).
|
Shile (Verified Customer) (01-31-2025) | 15463-1-AP SIN1 Rabbit PolyAb was working well in our Western blotting (1:500).
 |
