MAPRE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAPRE2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10364-1-AP

Synonyms

APC binding protein EB2, EB1, EB2, End binding protein 2, MAPRE2, RP1

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Validation Data Gallery

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  WB analysis of K-562 using 10364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse cells using 10364-1-AP

  Mouse keratinocyte showing MAPRE2 (#10364-1-AP, 1:100 dilution, with Alexa 594 labelled 2nd Ab) in red and alpha-tubulin (#66031-1-lg, 1:100 dilution, with Alexa 488 labelled 2nd Ab ) in green.

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells
• Positive IF detected in: mouse cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

10364-1-AP targets MAPRE2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPRE2 fusion protein Ag0416
• Full Name: microtubule-associated protein, RP/EB family, member 2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC007318
• Gene Symbol: MAPRE2
• Gene ID (NCBI): 10982
• RRID: AB_2141649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RP1 protein shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family and binds specifically to the C-terminal region of the APC protein. RP1 also binds directly to tubulin, both in vitro and in vivo. The function of RP1 is currently unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. It may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Publications

Species	Application	Title
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
mouse	WB	Exp Cell Res; MAPRE2 regulates the first meiotic progression in mouse oocytes.; Authors - Yuan-Yuan Li ; KD Validated