EB3 Polyclonal antibody

EB3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23974-1-AP

Synonyms

EB3, End binding protein 3, MAPRE3

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Validation Data Gallery

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  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23974-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 23974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 23974-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human skeletal muscle tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 23974-1-AP is EB3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EB3 fusion protein Ag21148
• Full Name: microtubule-associated protein, RP/EB family, member 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 30-36 kDa
• GenBank Accession Number: BC011557
• Gene Symbol: MAPRE3
• Gene ID (NCBI): 22924
• RRID: AB_2879385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	WB	Cell Tissue Res; Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress; Authors - Valentina Grande