Tested Applications
| Positive WB detected in | HeLa cells, U-937 cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12213-1-AP targets MARCH5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2806 Product name: Recombinant human MARCH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC015480 Sequence: MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRI Predict reactive species |
| Full Name | membrane-associated ring finger (C3HC4) 5 |
| Calculated Molecular Weight | 278 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa, 65-70 kDa |
| GenBank Accession Number | BC015480 |
| Gene Symbol | MARCH5 |
| Gene ID (NCBI) | 54708 |
| RRID | AB_10638602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NX47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MARCH5 antibody 12213-1-AP | Download protocol |
| IHC protocol for MARCH5 antibody 12213-1-AP | Download protocol |
| WB protocol for MARCH5 antibody 12213-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MARCH5 Polyclonal antibody (catalog number 12213-1-AP) has 5 total citations. These appear in journals including Clinical and Translational Medicine, Biochemical Pharmacology, Journal of Cell Biology, Biochimica et Biophysica Acta Molecular Cell Research, and eLife. The associated research fields span tumor-associated macrophages and neuroblastoma progression, cisplatin resistance in lung adenocarcinoma, peroxisome maintenance and pexophagy, aortic valve calcification and mitochondrial quality control, and BCL-2 family protein inhibitors in cancer.
| Species | Application | Title |
|---|---|---|
Clin Transl Med FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. | ||
Biochem Pharmacol Triflupromazine and tranylcypromine alleviate primary cisplatin resistance in lung adenocarcinoma by promoting LDHA-mediated AMBRA1 ubiquitination | ||
J Cell Biol The p97 ATPase and its adaptor UBXD8 maintain peroxisome pools by preventing pexophagy. | ||
Biochim Biophys Acta Mol Cell Res MARCH5 ameliorates aortic valve calcification via RACGAP1-DRP1 pathway associated mitochondrial quality control | ||
Elife Targeting the WSB2-NOXA axis in cancer cells for enhanced sensitivity to BCL-2 family protein inhibitors |
Reviews
What customers say
The single review reports a successful Western Blot experiment using HEK293 whole cell lysates. The sample was run on a denaturing acrylamide gel, transferred to nitrocellulose membrane, and incubated overnight with the antibody at a 1:1000 dilution. The reviewer rated the product 4 out of 5 stars, indicating a positive experience with clear detection in their assay.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tom (Verified Customer) (04-09-2021) | HEK293 whole cell lysates ran on denaturing acrylamide gel and trasnferred to nitrocellulose membrane. Incubated overnight with antibody.
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