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  • KD/KO Validated

MARCH5 Polyclonal antibody

MARCH5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12213-1-AP

Synonyms

MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153



Tested Applications

Positive WB detected inHeLa cells, U-937 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12213-1-AP targets MARCH5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARCH5 fusion protein Ag2806
Full Name membrane-associated ring finger (C3HC4) 5
Calculated molecular weight 278 aa, 31 kDa
Observed molecular weight 31 kDa, 65-70 kDa
GenBank accession numberBC015480
Gene symbol MARCH5
Gene ID (NCBI) 54708
RRIDAB_10638602
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for MARCH5 antibody 12213-1-APDownload protocol
IHC protocol for MARCH5 antibody 12213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (04-09-2021)

HEK293 whole cell lysates ran on denaturing acrylamide gel and trasnferred to nitrocellulose membrane. Incubated overnight with antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
MARCH5 Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:12213-1-AP)