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  • KD/KO Validated

MARCH5 Polyclonal antibody

MARCH5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12213-1-AP

Synonyms

MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12213-1-AP targets MARCH5 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARCH5 fusion protein Ag2806
Full Name membrane-associated ring finger (C3HC4) 5
Calculated molecular weight 278 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC015480
Gene symbol MARCH5
Gene ID (NCBI) 54708
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for MARCH5 antibody 12213-1-APDownload protocol
IHC protocol for MARCH5 antibody 12213-1-APDownload protocol
IP protocol for MARCH5 antibody 12213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (04-09-2021)

HEK293 whole cell lysates ran on denaturing acrylamide gel and trasnferred to nitrocellulose membrane. Incubated overnight with antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
MARCH5 Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:12213-1-AP)