|Positive WB detected in||HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12213-1-AP targets MARCH5 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||MARCH5 fusion protein Ag2806|
|Full Name||membrane-associated ring finger (C3HC4) 5|
|Calculated molecular weight||278 aa, 31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC015480|
|Gene ID (NCBI)||54708|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (04-09-2021)
HEK293 whole cell lysates ran on denaturing acrylamide gel and trasnferred to nitrocellulose membrane. Incubated overnight with antibody.