MARCKS Polyclonal antibody

MARCKS Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20661-1-AP

Synonyms

MARCS, MACS, 80K-L protein, 80KL, 80K L protein

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Validation Data Gallery

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  WB analysis using 20661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20661-1-AP (MARCKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20661-1-AP (MARCKS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20661-1-AP

  IP result of anti-MARCKS (IP:20661-1-AP, 4ug; Detection:20661-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human normal colon using 20661-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20661-1-AP (MARCKS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 20661-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MARCKS antibody (20661-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, IMR-32 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells, SK-N-SH cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

20661-1-AP targets MARCKS in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myristoylated alanine-rich protein kinase C substrate
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_002356
• Gene Symbol: MARCKS
• Gene ID (NCBI): 4082
• RRID: AB_2878719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Publications

Species	Application	Title
rat	WB	J Biol Chem; Oncoprotein DJ-1 interacts with mTOR complexes to effect transcription factor Hif1α-dependent expression of collagen I (α2) during renal fibrosis.; Authors - Falguni Das
human	WB	J Proteomics; Proteomic profile of human dental follicle stem cells and apical papilla stem cells.; Authors - Tong Lei
human	IP	Int J Oncol; Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma.; Authors - Nicholas J Eustace
human	WB,IF	Front Med (Lausanne); Enhanced understanding of cinnamaldehyde's therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect; Authors - Yueyang Sheng
human	WB	J Vet Med Sci; A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells; Authors - Jannatul Ferdous