Tested Applications
| Positive WB detected in | SH-SY5Y cells, HeLa cells, SK-N-SH cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20661-1-AP targets MARCKS in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myristoylated alanine-rich protein kinase C substrate |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | NM_002356 |
| Gene Symbol | MARCKS |
| Gene ID (NCBI) | 4082 |
| RRID | AB_2878719 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MARCKS antibody 20661-1-AP | Download protocol |
| IHC protocol for MARCKS antibody 20661-1-AP | Download protocol |
| IP protocol for MARCKS antibody 20661-1-AP | Download protocol |
| WB protocol for MARCKS antibody 20661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MARCKS antibody (Cat# 20661-1-AP) has 9 total citations published in journals including Nature Biomedical Engineering, Cell Death Discovery, Cell Reports, International Journal of Oncology, Journal of Biological Chemistry, Frontiers in Medicine, Journal of Proteomics, Journal of Veterinary Medical Science, and bioRxiv. Associated research fields span cancer proteomics/biomarkers, oocyte development, neural development, glioblastoma, renal fibrosis, osteoarthritis, dental stem cells, neuronal differentiation, and spinocerebellar ataxia.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Zona pellucida is required for oocyte actin cortex and oocyte-somatic cell interactions during oocyte growth. | ||
Cell Rep Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalon. | ||
Int J Oncol Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma. | ||
J Biol Chem Oncoprotein DJ-1 interacts with mTOR complexes to effect transcription factor Hif1α-dependent expression of collagen I (α2) during renal fibrosis. | ||
Front Med (Lausanne) Enhanced understanding of cinnamaldehyde's therapeutic potential in osteoarthritis through bioinformatics and mechanistic validation of its anti-apoptotic effect |
