CoraLite® Plus 488-conjugated MARCKS Polyclonal antibody

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• CoraLite® Plus 488-conjugated MARCKS Polyclonal antibody (CL488-20661)

MARCKS Polyclonal Antibody for FC (Intra)

Cat No. CL488-20661 Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

80K L, 80K L protein, 80KL, 80K-L protein, MACS

• 

  FC experiment of HeLa using CL488-20661

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MARCKS (CL488-20661) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20661 targets MARCKS in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myristoylated alanine-rich protein kinase C substrate
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_002356
• Gene Symbol: MARCKS
• Gene ID (NCBI): 4082
• RRID: AB_2919174
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29966
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Publications

What published studies show

MARCKS Polyclonal antibody (CoraLite® Plus 488), catalog number CL488-20661, has 1 verified citation. It appears in the American Journal of Respiratory and Critical Care Medicine (Impact Factor 21.7), published in November 2024. The associated research field is respiratory medicine and mesenchymal stromal cell therapy, specifically involving single-cell RNA sequencing to guide autologous preterm cord-derived cell therapy applications.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Am J Respir Crit Care Med; Single-Cell RNA Sequencing to Guide Autologous Preterm Cord Mesenchymal Stromal Cell-Therapy; Authors - Chanèle Cyr-Depauw