Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human small intestine tissue, rat adrenal gland tissue, human placenta tissue, rat stomach tissue, human stomach tissue, rat thyroid gland tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13515-1-AP targets MARVELD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4446 Product name: Recombinant human MARVELD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 365-558 aa of BC033689 Sequence: LWRHEAARRHREYMEQQEINEPSLSSKRKMCEMATSGDRQRDSEVNFKELRTAKMKPELLSGHIPPGHIPKPIVMPDYVAKYPVIQTDDERERYKAVFQDQFSEYKELSAEVQAVLRKFDELDAVMSRLPHHSESRQEHERISRIHEEFKKKKNDPTFLEKKERCDYLKNKLSHIKQRIQEYDKVMNWDVQGYS Predict reactive species |
| Full Name | MARVEL domain containing 2 |
| Calculated Molecular Weight | 558 aa, 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC033689 |
| Gene Symbol | MARVELD2 |
| Gene ID (NCBI) | 153562 |
| RRID | AB_2281702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4S9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MARVELD2 antibody 13515-1-AP | Download protocol |
| WB protocol for MARVELD2 antibody 13515-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MARVELD2 (Tricellulin) polyclonal antibody, catalog #13515-1-AP, has 7 total citations. These appear in J Clin Invest, Cell Rep, Int J Oral Sci, Microb Pathog, Tissue Cell, PLoS One, and Biomed Rep. Research fields include tight junction biology, Sjögren's syndrome hyposalivation, cochlear hair cell architecture, intestinal epithelial barrier modulation, PRRSV infection in piglets, electroreceptor cell biology, and pancreatic acinar cell regulation in acute pancreatitis.
| Species | Application | Title |
|---|---|---|
Int J Oral Sci Loss of tricellular tight junction tricellulin leads to hyposalivation in Sjögren's syndrome | ||
J Clin Invest Tricellulin deficiency affects tight junction architecture and cochlear hair cells. | ||
Cell Rep Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathways | ||
Microb Pathog Alterations in the expression level of visfatin in the lungs of piglets infected with PRRSV and its effect on PRRSV replication. | ||
Tissue Cell Papilla cells may guard the entrance to ampullary organs of Polyodon electroreceptors | ||
PLoS One Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon |
Reviews
What customers say
The product received a single 5-star review for Western Blot applications. The reviewer tested it on RWPE1, PC-3, and primary prostate epithelial cells at a 1:1000 dilution, reporting satisfactory performance with a clear band captured in the attached image. No written comments were provided beyond the rating and experimental details.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Beth (Verified Customer) (10-05-2017) |
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