MARVELD3 Polyclonal antibody

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• MARVELD3 Polyclonal antibody (25667-1-AP)

MARVELD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25667-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

MARVD3, MARVEL domain containing 3, MARVELD3, MRVLDC3

• 

  WB analysis of COLO 320 using 25667-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25667-1-AP (MARVELD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 25667-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25667-1-AP (MARVELD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 25667-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25667-1-AP (MARVELD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Caco-2 using 25667-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using MARVELD3 antibody (25667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25667-1-AP targets MARVELD3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22492

  Product name: Recombinant human MARVELD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-189 aa of BC013376

  Sequence: MEDPSGAREPRARPRERDPGRRPHPDQGRTHDRPRDRPGDPRRKRSSDGNRRRDGDRDPKRDQERDGNRDRNRDRERERERERDPDRGPRRDTHRDAGPRAGEHGVWEKPRQSRTRDGARGLTWDAAAPPGPAPWEAPEPPQPQRKGDPGRRRPESEPPSERYLPSTPRPGREEVEYYQSEAEGLLECH

• Full Name: MARVEL domain containing 3
• Calculated Molecular Weight: 401 aa, 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC013376
• Gene Symbol: MARVELD3
• Gene ID (NCBI): 91862
• RRID: AB_2880184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96A59
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356).

Publications

What published studies show

The MARVELD3 Polyclonal antibody (Cat# 25667-1-AP) has 8 citations published in journals including Int J Biol Macromol, Mol Med, ACS Omega, Bioorg Chem, Cell Signal, Food Chem Toxicol, PLoS One, and FEBS Open Bio. Research fields span oral and intestinal epithelial barrier biology, head and neck/esophageal cancer progression, radiation-induced ferroptosis, nanoplastic toxicity, and tight junction regulation.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Macromol; Molecular weight-dependent hyaluronic acid permeability and tight junction modulation in human buccal TR146 cell monolayers; Authors - Ha-Young Park
human	WB	Mol Med; Upregulation of PRRX2 by silencing Marveld3 as a protective mechanism against radiation-induced ferroptosis in skin cells; Authors - Jinming Cao
human	WB	ACS Omega; Physicochemical Attributes of Solid Hyaluronic Acid Technology Platform-Based Film Drive Rapid Oral Permeation and Barrier Enhancement via LKB1/CaMKKβ-AMPK Signaling.; Authors - Ha-Young Park
human	WB,IHC,IF,CoIP	Bioorg Chem; R-loop accumulation promotes OSCC malignant progression by upregulating MARVELD3 expression.; Authors - Xinyi Li
human	WB	Cell Signal; MarvelD3 regulates the radiosensitivity of Esophageal squamous cell carcinoma via the MYB/IFI6 axis.; Authors - Xi Wang ; KD Validated
human	WB,IF	Food Chem Toxicol; Polystyrene nanoplastics compromise oral epithelial barrier integrity and alter tight junction-related protein organization in TR146 human buccal epithelial cells.; Authors - Ha-Young Park