MAS1L Polyclonal antibody

• 

  WB analysis of HEK-293 using 20080-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20080-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20080-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAS1L antibody 20080-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

MAS1L Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20080-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MAS L, Mas related G protein coupled receptor MRG, MAS1L-Specific, MRG, MAS1-like

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Tested Applications

• Positive WB detected in: HEK-293 cells, COLO 320 cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Product Information

20080-1-AP targets MAS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: MAS1 oncogene-like
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_052967
• Gene Symbol: MAS1L
• Gene ID (NCBI): 116511
• RRID: AB_10665372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35410
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAS1L, also named MRG, belongs to the G-protein coupled receptor 1 family and Mas subfamily. The antibody is specific to MAS1L.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	J Transl Med; ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway; Authors - Feng Chen
	WB	Inflammation; ACE2 Expressed on Myeloid Cells Alleviates Sepsis-Induced Acute Liver Injury via the Ang-(1-7)-Mas Receptor Axis; Authors - Lei Liu
	WB	J Inflamm Res; The ACE2-Ang-(1‑7)-Mas Axis Modulates M1/M2 Macrophage Polarization to Relieve CLP-Induced Inflammation via TLR4-Mediated NF-кb and MAPK Pathways.; Authors - Hang Pan
	WB,IHC	ACS Omega; Azelaic Acid Regulates the Renin-Angiotensin System and Improves Colitis Based on Network Pharmacology and Experimentation; Authors - Yangjie Liao
rat	WB	Neural Plast; Electroacupuncture Improved Chronic Cerebral Hypoperfusion-Induced Anxiety-Like Behavior and Memory Impairments in Spontaneously Hypertensive Rats by Downregulating the ACE/Ang II/AT1R Axis and Upregulating the ACE2/Ang-(1-7)/MasR Axis.; Authors - Peipei Feng
rat	WB,IHC	Pharmacology; Canagliflozin Ameliorates Ventricular Remodeling through Apelin/Angiotensin-Converting Enzyme 2 Signaling in Heart Failure with Preserved Ejection Fraction Rats; Authors - Tingting Zhang