MASP1 Polyclonal antibody

MASP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21837-1-AP

Synonyms

Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5

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Validation Data Gallery

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  WB analysis of HeLa using 21837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21837-1-AP (MASP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 21837-1-AP is MASP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MASP1 fusion protein Ag16432
• Full Name: mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
• Calculated molecular weight: 728 aa, 82 kDa
• Observed molecular weight: 40-70 kDa
• GenBank accession number: BC106945
• Gene symbol: MASP1
• Gene ID (NCBI): 5648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Front Immunol; Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation; Authors - Binshan Zhang
human,mouse	WB,IHC,IP	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu ; KD Validated