Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67408-1-Ig targets MAT1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3076 Product name: Recombinant human MAT1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-395 aa of BC018359 Sequence: MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF Predict reactive species |
| Full Name | methionine adenosyltransferase I, alpha |
| Calculated Molecular Weight | 395 aa, 44 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC018359 |
| Gene Symbol | MAT1A |
| Gene ID (NCBI) | 4143 |
| RRID | AB_2882649 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00266 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAT1A antibody 67408-1-Ig | Download protocol |
| WB protocol for MAT1A antibody 67408-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAT1A antibody (Cat# 67408-1-Ig) has 4 total citations published in Cell Death Discovery, International Journal of Biological Macromolecules, Journal of Inherited Metabolic Disease, and Nature Communications. Research fields include ferroptosis resistance in hepatocellular carcinoma, methionine/one-carbon metabolism, MASH (metabolic dysfunction-associated steatohepatitis), intestinal flora remodeling, and bariatric surgery outcomes. Applications cited include WB and IF in human samples.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Activation of methionine metabolism mediated by HNF4α confers ferroptosis resistance in hepatocellular carcinoma. | ||
Int J Biol Macromol Atractylodes lancea (Thunb.) DC polysaccharide alleviates MASH by regulating the 1‑carbon cycle through intestinal flora remodelling. | ||
J Inherit Metab Dis Methionine Synthase Interacts With the Methionine Adenosyl-Transferase MATα2 and the DNA Methyltransferase DNMT3b in the Nucleus. | ||
Nat Commun Bariatric surgery resolves MASH by enhancing MAT1A-dependent one-carbon metabolism. |
