MAT2A Polyclonal antibody

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• MAT2A Polyclonal antibody (55309-1-AP)

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• KD/KO Validated

MAT2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55309-1-AP Copied!

Publications (31)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AMS2, EC:2.5.1.6, MAT 2, MATA2, MATII

• 

  WB analysis using 55309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55309-1-AP (MAT2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 55309-1-AP

  WB result of MAT2A antibody (55309-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-control and sh-MAT2A transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 55309-1-AP

  IP result of anti-MAT2A (IP:55309-1-AP, 4ug; Detection:55309-1-AP 1:500) with HepG2 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 55309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT2A antibody (55309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human kidney tissue, mouse liver tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55309-1-AP targets MAT2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: methionine adenosyltransferase II, alpha
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 47 kDa, 49 kDa
• GenBank Accession Number: NM_005911
• Gene Symbol: MAT2A
• Gene ID (NCBI): 4144
• RRID: AB_2881303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31153
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.

Publications

What published studies show

The MAT2A Polyclonal antibody (Cat# 55309-1-AP) has 31 citations. Journals include Nature, Science, Cell Metabolism, Nature Metabolism, Nature Communications, Molecular Cell, Advanced Science, Cell Death & Differentiation/Disease, Journal of Experimental Medicine, Cell Reports, FASEB Journal, Journal of Physiology, and others. Research fields span methionine/one-carbon metabolism, m6A RNA modification, mTORC1 signaling, ferroptosis, cancer, inflammation, and metabolism.

Automatically generated from the 31 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; Spermine is an endogenous iron chelator that inhibits ferroptosis; Authors - Man Li
mouse	WB	Science; Time-restricted feeding mitigates obesity through adipocyte thermogenesis; Authors - Chelsea Hepler
human	WB	Cell Metab; PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2; Authors - Cong Jiang
		Cell Metab; Methionine metabolism controls the B cell EBV epigenome and viral latency; Authors - Rui Guo
mouse,human	WB	Nat Metab; The SESAME complex regulates cell senescence through the generation of acetyl-CoA.; Authors - Wanping Chen ; KD Validated
mouse	WB,IF	Nat Commun; An RNA transmethylation pathway governs kidney nephrogenic potential; Authors - Harini Ramalingam