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  • KD/KO Validated

MAT2A Polyclonal antibody

MAT2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55309-1-AP

Synonyms

AdoMet synthase 2, AMS2, MAT 2, MAT II, MAT2A, MATA2, MATII, SAMS2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55309-1-AP targets MAT2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name methionine adenosyltransferase II, alpha
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_005911
Gene Symbol MAT2A
Gene ID (NCBI) 4144
RRIDAB_2881303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.

Protocols

Product Specific Protocols
WB protocol for MAT2A antibody 55309-1-APDownload protocol
IHC protocol for MAT2A antibody 55309-1-APDownload protocol
IF protocol for MAT2A antibody 55309-1-APDownload protocol
IP protocol for MAT2A antibody 55309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
  • KD Validated
humanWB,IF

Oxid Med Cell Longev

Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16.

Authors - Peng-Bo Chen
  • KD Validated
pigWB

Antioxidants (Basel)

M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide

Authors - Menghui Xu
mouseWB

FASEB J

Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.

Authors - Li Chen
  • KD Validated
mouseWB,IF

FASEB J

Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss.

Authors - Honglei Kang
  • KD Validated
ratWB

Environ Toxicol Pharmacol

Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.

Authors - Wenhua Kuang