MAT2B Polyclonal antibody

MAT2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 15952-1-AP

Synonyms

Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, Nbla02999, MSTP045, MATIIbeta, MAT II beta

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Validation Data Gallery

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  WB analysis of HepG2 using 15952-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15952-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15952-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15952-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15952-1-AP (MAT2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15952-1-AP

  IP result of anti-MAT2B (IP:15952-1-AP, 4ug; Detection:15952-1-AP 1:500) with HepG2 cells lysate 1000ug.

• 

  IHC staining of human lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15952-1-AP (MAT2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15952-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15952-1-AP (MAT2B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15952-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 15952-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAT2B antibody 15952-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung tissue, mouse lung tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 1 publications below

Product Information

15952-1-AP targets MAT2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAT2B fusion protein Ag8649
• Full Name: methionine adenosyltransferase II, beta
• Calculated Molecular Weight: 36 kDa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005218
• Gene Symbol: MAT2B
• Gene ID (NCBI): 27430
• RRID: AB_10637268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human		Ann N Y Acad Sci; PMID: 29722030; Authors - Holly M Brown-Borg
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia
chicken	WB	Sci Rep; In ovo injection of betaine alleviates corticosterone-induced fatty liver in chickens through epigenetic modifications.; Authors - Yun Hu
rat	WB	J Nutr Biochem; Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.; Authors - Nannan Zhao