Anti-Mouse MAdCAM1 Rabbit Recombinant Antibody

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• Anti-Mouse MAdCAM1 Rabbit Recombinant Antibody (98444-1-RR)

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  FC experiment of Transfected HEK-293T using 98444-1-RR

  1x10^6 HEK-293T or MAdCAM1-transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse MAdCAM1 Rabbit RecAb (98444-1-RR, Clone: 242902D6) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 98444-1-RR

  1x10^6 HEK-293T or MAdCAM1-transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse MAdCAM1 Rabbit RecAb (98444-1-RR, Clone: 242902D6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

MAdCAM1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98444-1-RR

Clone No.242902D6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Adhesion molecule immunoglobulin-like domain-containing protein, MAdCAM-1

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98444-1-RR targets MAdCAM1 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2010

  Product name: Recombinant Mouse MAdCAM-1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-364 aa of NP_038619

  Sequence: QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGQVTNLYVPGQVTPNSSS

• Full Name: mucosal vascular addressin cell adhesion molecule 1
• Calculated Molecular Weight: 44kDa
• GenBank Accession Number: NP_038619
• Gene Symbol: Madcam1
• Gene ID (NCBI): 17123
• RRID: AB_3746082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.