MBD2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86393-3-RR (same clone as 86393-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86393-3-RR (MBD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86393-3-PBS in a different storage buffer formulation.

MBD2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86393-3-PBS

Clone No.250339G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Demethylase, DMTase, Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, NY CO 41

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Product Information

86393-3-PBS targets MBD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: methyl-CpG binding domain protein 2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-50 kDa
• GenBank Accession Number: NM_003927
• Gene Symbol: MBD2
• Gene ID (NCBI): 8932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBB5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing (PMID:9774669). MBD2 belongs to a highly conserved member of the MBD protein family, is considered a reader of DNA methylation and drives the crosstalk between DNA methylation and histone deacetylation (PMID: 40089655). MBD2 drives breast cancer progression through the modulation of epithelial-to-mesenchymal transition (PMID: 38556549).