MCM10 Polyclonal antibody

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• MCM10 Polyclonal antibody (12251-1-AP)

MCM10 Polyclonal Antibody for WB, ELISA

Cat No. 12251-1-AP Copied!

Publications (19)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

CNA43, DNA43, HsMCM10, PRO2249, Protein MCM10 homolog

• 

  WB analysis using 12251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12251-1-AP (MCM10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12251-1-AP (MCM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HUVEC cells, Ramos cells, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12251-1-AP targets MCM10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2899

  Product name: Recombinant human MCM10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 259-611 aa of BC009108

  Sequence: MDLPTCGARNLKQHLAKASASGIMGSPKPAIKSISASALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKWERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK

• Full Name: minichromosome maintenance complex component 10
• Calculated Molecular Weight: 874 aa, 97 kDa
• Observed Molecular Weight: 97-105 kDa
• GenBank Accession Number: BC009108
• Gene Symbol: MCM10
• Gene ID (NCBI): 55388
• RRID: AB_2266259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L590
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM10, also named as HsMCM10, belongs to the MCM10 family. It acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, it plays a role in preventing DNA damage during replication and is essential for the integrity of the RECQ4-MCM replicative helicase complex. It mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

Publications

What published studies show

MCM10 Polyclonal antibody (Cat# 12251-1-AP) has 19 citations across 16 journals, including Nature Communications, Cancer Discovery, EMBO Journal, Cell Reports, Journal of Cell Biology, Journal of Biological Chemistry, Oncotarget, and eLife. Research fields span DNA replication, genome stability, cell cycle regulation, DNA damage response, and cancer biology (breast, lung, and liver cancers).

Automatically generated from the 19 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Discov; DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.; Authors - Mazurek Anthony A
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.; Authors - Zhihua Kang
human	WB,IHC	Adv Sci (Weinh); Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage; Authors - Jiaxi Zhang
human	WB	EMBO J; MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.; Authors - Xu Xiaohua X
human	WB	Cell Rep; Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.; Authors - Jiin-Tarng Wang