MCM10 Polyclonal antibody

MCM10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12251-1-AP

Synonyms

CNA43, DNA43, HsMCM10, MCM10, PRO2249, Protein MCM10 homolog



Tested Applications

Positive WB detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12251-1-AP targets MCM10 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCM10 fusion protein Ag2899
Full Name minichromosome maintenance complex component 10
Calculated Molecular Weight 874 aa, 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberBC009108
Gene Symbol MCM10
Gene ID (NCBI) 55388
RRIDAB_2266259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM10, also named as HsMCM10, belongs to the MCM10 family. It acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, it plays a role in preventing DNA damage during replication and is essential for the integrity of the RECQ4-MCM replicative helicase complex. It mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

Protocols

Product Specific Protocols
WB protocol for MCM10 antibody 12251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Commun

DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.

Authors - Jing Zhang
humanWB

Nat Commun

BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.

Authors - Zhihua Kang
humanWB

Cancer Discov

DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.

Authors - Mazurek Anthony A
humanWB

EMBO J

MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

Authors - Xu Xiaohua X
human

J Cell Biol

Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.

Authors - Divya Achuthankutty
humanWB

Cell Rep

Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.

Authors - Jiin-Tarng Wang