MCM10 Polyclonal antibody

• 

  WB analysis of Ramos using 12251-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 12251-1-AP (MCM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCM10 Polyclonal Antibody for WB, ELISA

Cat No. 12251-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

CNA43, DNA43, HsMCM10, MCM10, PRO2249, Protein MCM10 homolog

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Tested Applications

• Positive WB detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IF: See 1 publications below

Product Information

12251-1-AP targets MCM10 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2899

  Product name: Recombinant human MCM10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 259-611 aa of BC009108

  Sequence: MDLPTCGARNLKQHLAKASASGIMGSPKPAIKSISASALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKWERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK

• Full Name: minichromosome maintenance complex component 10
• Calculated Molecular Weight: 874 aa, 97 kDa
• Observed Molecular Weight: 97-105 kDa
• GenBank Accession Number: BC009108
• Gene Symbol: MCM10
• Gene ID (NCBI): 55388
• RRID: AB_2266259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L590
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM10, also named as HsMCM10, belongs to the MCM10 family. It acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, it plays a role in preventing DNA damage during replication and is essential for the integrity of the RECQ4-MCM replicative helicase complex. It mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.; Authors - Zhihua Kang
human	WB	Cancer Discov; DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.; Authors - Mazurek Anthony A
human	WB	EMBO J; MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.; Authors - Xu Xiaohua X
human		J Cell Biol; Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.; Authors - Divya Achuthankutty
human	WB	Cell Rep; Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.; Authors - Jiin-Tarng Wang