• Featured Product
  • KD/KO Validated

MCM2 Monoclonal antibody

MCM2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66204-1-Ig
Clone No.1F4D10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells, A431 cells, 4T1 cells, A549 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inHuman tonsillitis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66204-1-Ig targets MCM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM2 fusion protein Ag0798 Predict reactive species
Full Name minichromosome maintenance complex component 2
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
RRIDAB_2881595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MCM2 antibody 66204-1-IgDownload protocol
IHC protocol for MCM2 antibody 66204-1-IgDownload protocol
IF protocol for MCM2 antibody 66204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Death Discov

MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling

Authors - Xin Zhou
Loading...