MCM8 Recombinant monoclonal antibody, PBS Only

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• MCM8 Recombinant monoclonal antibody, PBS Only (87212-1-PBS)

• 

  WB analysis using 87212-1-RR (same clone as 87212-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87212-1-RR (MCM8 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87212-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87212-1-RR (same clone as 87212-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87212-1-RR (MCM8 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87212-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 87212-1-RR (same clone as 87212-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MCM8 antibody (87212-1-RR, Clone: 252460F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87212-1-PBS in a different storage buffer formulation.

MCM8 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87212-1-PBS

Clone No.252460F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C20orf154, DNA helicase MCM8, EC:3.6.4.12, MCM 8, REC

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Product Information

87212-1-PBS targets MCM8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8430

  Product name: Recombinant human MCM8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 380-735 aa of BC008830

  Sequence: SGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM

• Full Name: minichromosome maintenance complex component 8
• Calculated Molecular Weight: 880 aa, 98 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC008830
• Gene Symbol: MCM8
• Gene ID (NCBI): 84515
• RRID: AB_3745410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UJA3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis.