MCM8 Recombinant monoclonal antibody

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• MCM8 Recombinant monoclonal antibody (87212-1-RR)

• 

  WB analysis using 87212-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87212-1-RR (MCM8 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87212-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87212-1-RR (MCM8 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 87212-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MCM8 antibody (87212-1-RR, Clone: 252460F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MCM8 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87212-1-RR

Clone No.252460F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

C20orf154, DNA helicase MCM8, EC:3.6.4.12, MCM 8, REC

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Tested Applications

• Positive WB detected in: HCT 116 cells, NIH/3T3 cells, SW 480 cells, U2OS cells, HEK-293T cells, RAW 264.7 cells, HSC-T6 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87212-1-RR targets MCM8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8430

  Product name: Recombinant human MCM8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 380-735 aa of BC008830

  Sequence: SGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM

• Full Name: minichromosome maintenance complex component 8
• Calculated Molecular Weight: 880 aa, 98 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC008830
• Gene Symbol: MCM8
• Gene ID (NCBI): 84515
• RRID: AB_3745410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UJA3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis.