MCM9 Recombinant monoclonal antibody

• 

  WB analysis using 83587-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83587-1-RR (MCM9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MCM9 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83587-1-RR

Clone No.240252E9

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

C6orf61, dJ329L24.1, dJ329L24.3, FLJ13942, FLJ20170, FLJ56845, hMCM9, MCM9, MCMDC1

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83587-1-RR targets MCM9 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4108

  Product name: Recombinant human MCM9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC031658

  Sequence: MNSDQVTLVGQVFESYVSEYHKNDILLILKERDEDAHYPVVVNAMTLFETNMEIGEYFNMFPSEVLTIFDSALRRSALTILQSLSQPEAVSMKQNLHARISGLPVCPELVREHIPKTKDVGHFLSVTGTVIRTSLVKVLEFERDYMCNKCKHVFVIKADFEQYYTFCRPSSCPSLESCDSSKFTCLSGLSSSPTRCRDYQEIKIQEQVQRLSVGSIPRSMKVILEDDLVDSCKSGDDLTIYGIVMQRWKPFQQDVRCEVEIVLKANYIQVNNEQSSGIIMDEEVQKEFEDFWEYYKSDPFAGRNVILASLCPQVFGMYLVKLAVAMVL

• Full Name: minichromosome maintenance complex component 9
• Calculated Molecular Weight: 1143 aa, 127 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC031658
• Gene Symbol: MCM9
• Gene ID (NCBI): 254394
• RRID: AB_3671201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NXL9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a protein that belongs minichromosome maintenance (MCM) family, which are known to be essential for initiation of DNA replication. MCM9 binds to chromatin in an ORC-dependent manner and is required for the recruitment of the MCM2-7 helicase onto chromatin. Human MCM9 proteins consist of 1143 amino acids with a molecular weight of approximately 130 kDa.