FcZero-rAb® APC Anti-Human MCP-1/CCL2 Rabbit Recombinant Antibody

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• FcZero-rAb® APC Anti-Human MCP-1/CCL2 Rabbit Recombinant Antibody (APC-FcA98044)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

MCP-1/CCL2 FcZero-rAb^® Recombinant Antibody for FC (Intra)

Cat No. APC-FcA98044 Copied!

Clone No. 230270A7

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CCL2, MCP1, MCP-1, C C motif chemokine 2, C-C motif chemokine 2

• 

  FC experiment of THP-1 using APC-FcA98044

  1x10^6 untreated or PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 5 ul APC Anti-Human MCP-1/CCL2 Rabbit RecAb (APC-FcA98044, Clone: 230270A7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: PMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98044 targets MCP-1/CCL2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg31375

  Product name: Recombinant Human MCP-1/CCL2 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 24-99 aa of BC009716

  Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC009716
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 6347
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13500
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, is a chemokine that regulates monocyte and macrophage migration and infiltration. In addition to monocytes and macrophages, MCP-1 can also serve as a chemoattractant for T cells, B cells, NK cells, dendritic cells, and neutrophils. MCP-1 expression is typically induced by the release of inflammatory cytokines and other stimuli from sites of tissue injury and infection. It in turn promotes the extravasation of immune cells across blood vessel walls and their subsequent infiltration of the infection site. It may also play a role in macrophage polarization as well as the differentiation of monocytes into dendritic cells, MSDCs, and osteoclasts. Increased MCP-1 expression has been linked to inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and multiple sclerosis. (PMID: 19441883, 31921102)