Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue, HepG2 cells, rat lung tissue, L02 cells |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15462-1-AP targets MDH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, caenorhabditis elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7750 Product name: Recombinant human MDH2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-338 aa of BC001917 Sequence: MLSALARPVSAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK Predict reactive species |
| Full Name | malate dehydrogenase 2, NAD (mitochondrial) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC001917 |
| Gene Symbol | MDH2 |
| Gene ID (NCBI) | 4191 |
| RRID | AB_2878143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MDH2 antibody 15462-1-AP | Download protocol |
| IHC protocol for MDH2 antibody 15462-1-AP | Download protocol |
| WB protocol for MDH2 antibody 15462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MDH2 Polyclonal antibody (Cat# 15462-1-AP) has 41 citations. These appear in high-impact journals including Nature, Cell Metabolism, Science Translational Medicine, Molecular Cell, Cell Reports, Journal of Hepatology, and Nature Communications, among others. Research fields span mitochondrial metabolism and the TCA cycle, post-translational modifications (succinylation, lactylation, β-hydroxybutyrylation), cancer biology (hepatocellular, renal, colorectal, breast, glioblastoma), ferroptosis, heart regeneration, neurodegeneration, and metabolic regulation. Most studies applied the antibody for Western blot in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Cell Metab Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2 | ||
Drug Resist Updat A tumor-derived lactate/ENO1 lactylation feedback loop facilitates osimertinib resistance of lung adenocarcinoma. | ||
Mol Neurodegener Mitovesicles secreted into the extracellular space of brains with mitochondrial dysfunction impair synaptic plasticity | ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. |
Reviews
What customers say
The single review rates this antibody 4 out of 5 stars for Western Blot at a 1:1000 dilution in HeLa cells. The reviewer found it a good antibody that specifically detects MDH2 around 34 kDa, though some nonspecific bands were also observed. Overall, the antibody performed well for its intended target despite minor background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Süleyman (Verified Customer) (02-06-2022) | It is good antibody but some unspecific bands, but MDH2 can be detected specifically around 34 kDa.
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