MDH2 Polyclonal antibody

MDH2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15462-1-AP

Synonyms

M MDH, MDH, MDH2, MOR1



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

15462-1-AP targets MDH2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Caenorhabditis elegans, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDH2 fusion protein Ag7750
Full Name malate dehydrogenase 2, NAD (mitochondrial)
Calculated molecular weight 36 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001917
Gene symbol MDH2
Gene ID (NCBI) 4191
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MDH2 antibody 15462-1-APDownload protocol
IHC protocol for MDH2 antibody 15462-1-APDownload protocol
IP protocol for MDH2 antibody 15462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Caenorhabditis elegansWB

Int J Radiat Biol

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

Authors - Yongyan Sun
humanWB

J Ethnopharmacol

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Authors - Li Wu
humanWB

FEBS Lett

Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase.

Authors - Virginie F Rhein

Mol Cell Biol

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Authors - Olga Zurita Rendón
mouseWB

Biochim Biophys Acta Mol Basis Dis

Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.

Authors - Qianqian Guo
mouseWB

iScience

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Authors - Tirthankar Sinha