MDM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MDM2 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 27883-1-AP

Synonyms

Double minute 2 protein, hdm2, HDMX, MDM2, Oncoprotein Mdm2, p53 binding protein Mdm2

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Validation Data Gallery

• 

  WB analysis using 27883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27883-1-AP (MDM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 27883-1-AP

  WB result of MDM2 antibody (27883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM2 transfected A431 cells.

• 

  FC experiment of HeLa using 27883-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MDM2 (27883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: MCF-7 cells, A431 cells, T-47D cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

27883-1-AP targets MDM2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MDM2 fusion protein Ag27255
• Full Name: Mdm2 p53 binding protein homolog (mouse)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: NM_001145337.2
• Gene Symbol: MDM2
• Gene ID (NCBI): 4193
• RRID: AB_2881003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the cellular localization of MDM2?

MDM2 is predominantly localized in the nucleoplasm.

What is the tissue specificity of MDM2?

MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain 

isoforms only express in cancerous cells and not normal tissue.

With what other molecules does MDM2 interact?

Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional 

activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to 

MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.

What competes with MDM2 activity?

Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.

What is the role of ubiquitination in MDM2 activity?

While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination 

mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of 

p53.

How is MDM2 associated with cancer?

MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in 

promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development 

and growth.

What is the human ortholog of MDM2?

Human double-minute type 2 (HDM2)

In what cellular circumstances does the p53-MDM2 complex dissociate?

Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also 

prevents p53-MDM2 interaction.

Where is the gene located?

Hdm2 is located on human 12q15.

Mdm2 is located on murine 10 D2.

PMIDs: 24309898, 29584707, 9840926, 28714398

Publications

Species	Application	Title
rat	WB	J Clin Invest; ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.; Authors - Hidemichi Kouzu
human	WB	Bioorg Chem; Design, synthesis and biological evaluation of novel pyrrolidone-based derivatives as potent p53-MDM2 inhibitors.; Authors - Dongjuan Si
human	WB	Mol Cell Biol; USP49-mediated histone H2B deubiquitination regulates HCT116 cell proliferation through MDM2-p53 axis.; Authors - Lei Shi
human	WB	Curr Oncol; Regulatory and Interacting Partners of PDLIM7 in Thyroid Cancer; Authors - Kristiana Rood
human	WB	Curr Res Neurobiol; The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics; Authors - Chantal A Pileggi
human	WB,IHC,IF	Oncogene; Imbalance in ALR ubiquitination accelerates the progression of nonalcoholic steatohepatitis to hepatocellular carcinoma; Authors - Mingzhe Zheng ; KD Validated

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