MED15 Polyclonal antibody

• Featured Product
• KD/KO Validated

MED15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 11566-1-AP

Synonyms

Mediator of RNA polymerase II transcription subunit 15, CTG7A, CTG repeat protein 7a, CAG7A, ARC105

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Validation Data Gallery

• 

  WB analysis of K-562 using 11566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 11566-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 11566-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11566-1-AP

  IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug.

• 

  IHC staining of human prostate cancer using 11566-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 11566-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, SMMC-7721 cells, HuH-7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• ChIP: See 5 publications below

Product Information

11566-1-AP targets MED15 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED15 fusion protein Ag2143
• Full Name: mediator complex subunit 15
• Calculated Molecular Weight: 788 aa, 87 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC017110
• Gene Symbol: MED15
• Gene ID (NCBI): 51586
• RRID: AB_2141975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

Publications

Species	Application	Title
		Mol Cell; Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.; Authors - Nilay Shah
mouse	WB	Cell Rep; Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.; Authors - Min Xia
human	WB,IHC,IF	Int J Cancer; MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.; Authors - Zaki Shaikhibrahim ; KD Validated
human, mouse	ChIP	PLoS Genet; Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.; Authors - Naomi Shomer
human	WB	J Biol Chem; Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes.; Authors - Tsutsui Taiki T
human	WB, IHC, IF	Am J Pathol; Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma.; Authors - Zaki Shaikhibrahim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

between 75-100

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED15 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:11566-1-AP)

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