Tested Applications
| Positive WB detected in | K-562 cells, SMMC-7721 cells, HuH-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11566-1-AP targets MED15 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2143 Product name: Recombinant human MED15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC017110 Sequence: DPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHSMAVVSTATPQTQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQ Predict reactive species |
| Full Name | mediator complex subunit 15 |
| Calculated Molecular Weight | 788 aa, 87 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC017110 |
| Gene Symbol | MED15 |
| Gene ID (NCBI) | 51586 |
| RRID | AB_2141975 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RN5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MED15 antibody 11566-1-AP | Download protocol |
| IHC protocol for MED15 antibody 11566-1-AP | Download protocol |
| IP protocol for MED15 antibody 11566-1-AP | Download protocol |
| WB protocol for MED15 antibody 11566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is anti-MED15 (Cat# 11566-1-AP), with a total of 24 citations. Publications appear in journals including Nature Communications, Molecular Cell, Nucleic Acids Research, Cell Reports, Cell Death & Discovery, Journal of Biological Chemistry, PLoS Genetics, FASEB Journal, International Journal of Cancer, and American Journal of Pathology. Research fields span Mediator complex transcriptional regulation, cancer biology (prostate, renal cell, bladder, and head and neck cancers), EMT/metastasis, adipogenesis, lipid metabolism, and epigenetic regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun The mechanism of YAP/TAZ transactivation and dual targeting for cancer therapy | ||
Mol Cell Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors | ||
Mol Cell Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. | ||
Nucleic Acids Res PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts | ||
Cell Death Discov MED15 is upregulated by HIF-2α and promotes proliferation and metastasis in clear cell renal cell carcinoma via activation of SREBP-dependent fatty acid synthesis |
Reviews
What customers say
Both reviewers used this antibody for Western Blotting and reported positive results. One user (mouse tissue, 1:2000 dilution) gave it 5 stars, describing it as highly specific and strong, and plans to test it for IP and ChIP. The other (embryo heart tissue, 1:500 dilution) gave it 4 stars, observing a band between 75–100 kDa. Overall, users find it reliable and specific for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ivan (Verified Customer) (05-30-2025) | Works very well. Highly specific and quite strong. WB: 1/2000 and diluted ECL. I will try for IP and ChIP.
|
SITING (Verified Customer) (04-02-2021) | between 75-100
 |
