Tested Applications
| Positive WB detected in | human heart tissue, HEK-293 cells |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16787-1-AP targets MED30 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10221 Product name: Recombinant human MED30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC008226 Sequence: MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGTYQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN Predict reactive species |
| Full Name | mediator complex subunit 30 |
| Calculated Molecular Weight | 178 aa, 20 kDa |
| Observed Molecular Weight | 20-25 kDa |
| GenBank Accession Number | BC008226 |
| Gene Symbol | MED30 |
| Gene ID (NCBI) | 90390 |
| RRID | AB_2142439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MED30 antibody 16787-1-AP | Download protocol |
| WB protocol for MED30 antibody 16787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MED30 antibody (Cat# 16787-1-AP) has 6 total citations. Publications appear in Nature Genetics, Journal of Autoimmunity, Scientific Reports, PLoS One, Anticancer Research, and Annals of Diagnostic Pathology. Research fields span cancer biology (pancreatic, gastric, bladder, and renal cell carcinoma), autoimmune disease (systemic sclerosis), and gene regulation (MYC oncogene, CRISPR screening). Applications include WB, IHC, and immune complex analysis in human samples.
| Species | Application | Title |
|---|---|---|
Nat Genet Interaction between MED12 and ΔNp63 activates basal identity in pancreatic ductal adenocarcinoma | ||
J Autoimmun Immune complexome analysis of a rich variety of serum immune complexes identifies disease-characteristic immune complex antigens in systemic sclerosis | ||
Sci Rep Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulator. | ||
Anticancer Res The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
| ||
Ann Diagn Pathol The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.
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Reviews
What customers say
The single review for this antibody rates it 4 out of 5 stars. The user tested it by Western Blot on heart tissue and reported that it successfully detects a MED30 band at approximately 22 kD. Overall, the feedback is positive, confirming the antibody's ability to recognize its target protein at the expected molecular weight in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-25-2020) | This antibody detects a MED30 band at around 22kD.
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