MEN1 Polyclonal antibody

MEN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15159-1-AP

Synonyms

SCG2, Menin 1, Menin, MEAI

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Validation Data Gallery

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  WB analysis of HepG2 using 15159-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15159-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15159-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15159-1-AP

  IP result of anti-MEN1 (IP:15159-1-AP, 4ug; Detection:15159-1-AP 1:500) with HEK-293 cells lysate 1320 ug.

• 

  IF Staining of HeLa using 15159-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15159-1-AP (MEN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15159-1-AP targets MEN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEN1 fusion protein Ag7253
• Full Name: multiple endocrine neoplasia I
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC002544
• Gene Symbol: MEN1
• Gene ID (NCBI): 4221
• RRID: AB_2250607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice

Publications

Species	Application	Title
human	WB	Cell Rep; DOT1L/H3K79me2 represses HIV-1 reactivation via recruiting DCAF1; Authors - Fenfei Liang

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