MEPCE Polyclonal antibody

MEPCE Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11526-1-AP

Synonyms

Bin3 homolog, MEPCE, methylphosphate capping enzyme



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11526-1-AP targets MEPCE in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEPCE fusion protein Ag2092
Full Name methylphosphate capping enzyme
Calculated molecular weight 689 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC018935
Gene symbol MEPCE
Gene ID (NCBI) 56257
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEPCE is a Component of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.

Protocols

Product Specific Protocols
WB protocol for MEPCE antibody 11526-1-APDownload protocol
IHC protocol for MEPCE antibody 11526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA Biol

7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb.

Authors - Eilebrecht Sebastian S
humanWB

Nucleic Acids Res

7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.

Authors - Eilebrecht Sebastian S
zebrafishWB,IP

Proc Natl Acad Sci U S A

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.

Authors - Barboric Matjaz M
humanWB

Dev Cell

Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation.

Authors - Kamaleldin E Elagib

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

MIAO (Verified Customer) (09-17-2020)

good Ab with nice WB signal

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1K
  • Cell Tissue Type: HCT cell