|Positive WB detected in||HeLa cells, K-562 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11526-1-AP targets MEPCE in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MEPCE fusion protein Ag2092|
|Full Name||methylphosphate capping enzyme|
|Calculated molecular weight||689 aa, 74 kDa|
|Observed molecular weight||74 kDa|
|GenBank accession number||BC018935|
|Gene ID (NCBI)||56257|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MEPCE is a Component of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.
7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb.
Nucleic Acids Res
7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.
Proc Natl Acad Sci U S A
7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.
Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
MIAO (Verified Customer) (09-17-2020)
good Ab with nice WB signal