Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11526-1-AP targets MEPCE in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2092 Product name: Recombinant human MEPCE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 369-689 aa of BC018935 Sequence: SSKSEAGARGGGQGSKEKGRGSWGGRHHHHHPLPAAGFKKQQRKFQYGNYCKYYGYRNPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLSIACKWGPSRMVGLDIDSRLIHSARQNIRHYLSEELRLPPQTLEGDPGAEGEEGTTTVRKRSCFPASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDDLVEAQTPEYDVVLCLSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYGKRKTLTETIYKNYYRIQLKPEQFSSYLTSPDVGFSSYELVATPHNTSKGFQRPVYLFHKARSPSH Predict reactive species |
| Full Name | methylphosphate capping enzyme |
| Calculated Molecular Weight | 689 aa, 74 kDa |
| Observed Molecular Weight | 74 kDa |
| GenBank Accession Number | BC018935 |
| Gene Symbol | MEPCE |
| Gene ID (NCBI) | 56257 |
| RRID | AB_2143568 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L2J0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEPCE is a Component of the 7SK snRNP complex, and a S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, which leads to its stabilization.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MEPCE antibody 11526-1-AP | Download protocol |
| WB protocol for MEPCE antibody 11526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MEPCE polyclonal antibody (Cat# 11526-1-AP) has 4 citations. These appear in Nucleic Acids Research, Proceedings of the National Academy of Sciences, Developmental Cell, and RNA Biology. The research fields span transcription regulation, 7SK snRNP/P-TEFb biology, alternative splicing, and megakaryocyte morphogenesis, with studies conducted in human and zebrafish models using WB and IP applications.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation. | ||
Proc Natl Acad Sci U S A 7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development. | ||
Dev Cell Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation. | ||
Reviews
What customers say
One reviewer rated this antibody 4 out of 5 stars, using it at a 1:1,000 dilution on HCT cells for Western Blot. They reported "good Ab with nice WB signal," indicating satisfactory performance in their application. Overall, the single review reflects positive feedback on the antibody's signal quality in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MIAO (Verified Customer) (09-17-2020) | good Ab with nice WB signal
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