MEST Monoclonal antibody, PBS Only

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  WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

MEST Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60263-1-PBS

Clone No.1A4A1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1

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Product Information

60263-1-PBS targets MEST in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MEST fusion protein Ag11929
• Full Name: mesoderm specific transcript homolog (mouse)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC011908
• Gene Symbol: MEST
• Gene ID (NCBI): 4232
• RRID: AB_2881384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5EB52
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.