METAP2 Polyclonal antibody

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• METAP2 Polyclonal antibody (17040-1-AP)

METAP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 17040-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MAP 2, MAP2, MetAP 2, METAP2, Methionine aminopeptidase 2, methionyl aminopeptidase 2, MNPEP, p67, p67eIF2, Peptidase M 2

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  WB analysis using 17040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of MCF-7 using 17040-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 17040-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 17040-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 17040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17040-1-AP (METAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 17040-1-AP

  IP result of anti-METAP2 (IP:17040-1-AP, 4ug; Detection:17040-1-AP 1:300) with MCF-7 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 17040-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 17040-1-AP (METAP2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 17040-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17040-1-AP (METAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, mouse pancreas tissue, mouse testis tissue
• Positive IP detected in: MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17040-1-AP targets METAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10564

  Product name: Recombinant human METAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 132-478 aa of BC013782

  Sequence: GQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

• Full Name: methionyl aminopeptidase 2
• Calculated Molecular Weight: 478 aa, 53 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC013782
• Gene Symbol: METAP2
• Gene ID (NCBI): 10988
• RRID: AB_2144162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50579
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MetAP2, also known as MAP2, P67EIF2 or p67, is one of the three known methionine aminopeptidases responsible for the removal of the N-terminal translation initiator methionine from newly synthesized proteins, which is a critical step in protein maturation (PMID: 27692546, 16568094). The enzyme binds two cobalt or manganese ions in its active site (PMID: 11524009, 12718546). As higher MetAP2 expression was observed in tumor (mesothelioma, neuroblastoma, and colorectal carcinoma) cells compared with normal cells, the enzyme serves as a target for anti-angiogenic compounds of natural origin, such as fumagillin and ovalicin (PMID: 11485930, 15927358, 15102683, 9224570). Selective inhibition of MetAP2 stops vascularization and tumor growth in animal models (PMID: 12118091). MetAP2 has 3 isoforms with molecular mass of 50-53 kDa and apparent molecular mass 67 kD.

Publications

What published studies show

The METAP2 Polyclonal antibody (Cat# 17040-1-AP) has a total of 2 citations. These appear in Molecular Cell and Science Advances. The cited research covers ribosome biology (decoding expansion segment function) and cotranslational histone modification mechanisms involving MetAP1 and NatD, reflecting applications in protein maturation and epigenetic regulation studies.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell; Decoding the Function of Expansion Segments in Ribosomes.; Authors - Kotaro Fujii
human	WB	Sci Adv; Mechanism of cotranslational modification of histones H2A and H4 by MetAP1 and NatD.; Authors - Denis Yudin ; KD Validated