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  • KD/KO Validated

METTL16 Polyclonal antibody

METTL16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19924-1-AP

Synonyms

METT10D, METTL16



Tested Applications

Positive WB detected inHEK-293 cells, rat testis tissue, HeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19924-1-AP targets METTL16 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL16 fusion protein Ag13889
Full Name methyltransferase 10 domain containing
Calculated Molecular Weight 562 aa, 64 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001213
Gene Symbol METT10D
Gene ID (NCBI) 79066
RRIDAB_10639364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methyltransferase-like protein 16 (METTL16) is a human RNA methyltransferase that installs m6A marks on U6 small nuclear RNA (U6 snRNA) and S-adenosylmethionine (SAM) synthetase pre-mRNA. METTL16 is considered a protective gene, suppressing the development of OC, HCC, and endocrine system tumors (PMID: 33237039, PMID: 33133142, PMID: 34187307). However, the high expression of METTL16 has been linked with a poor survival rate in patients with breast cancer (PMID: 33362863). METTL16 has 2 isoforms with molecular weights of 64 and 26 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL16 antibody 19924-1-APDownload protocol
IHC protocol for METTL16 antibody 19924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.

Authors - Deguan Lv
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
humanWB

Oxid Med Cell Longev

Methylation Pattern Mediated by m6A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma.

Authors - Feng Jiang
humanWB

J Biol Chem

m6A demethylation of cytidine deaminase APOBEC3B mRNA orchestrates arsenic-induced mutagenesis.

Authors - Ming Gao
humanWB,IHC

Anal Cell Pathol (Amst)

METTL16 Inhibits the Malignant Progression of Epithelial Ovarian Cancer through the lncRNA MALAT1/β-Catenin Axis

Authors - Changshu Li
  • KD Validated
humanIHC

J Coll Physicians Surg Pak

Clinical Significance of Methyltransferase-like 16 Expression in Epithelial Ovarian Cancer

Authors - Changshu Li