METTL11A Polyclonal antibody

METTL11A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17184-1-AP

Synonyms

methyltransferase like 11A, EC:2.1.1.244, C9orf32, Alpha N-terminal protein methyltransferase 1A, AD-003

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Validation Data Gallery

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  WB analysis using 17184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17184-1-AP (METTL11A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 17184-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17184-1-AP (METTL11A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells, HepG2 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17184-1-AP targets METTL11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL11A fusion protein Ag10967
• Full Name: methyltransferase like 11A
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 16 kDa, 26 kDa
• GenBank Accession Number: BC033234
• Gene Symbol: METTL11A
• Gene ID (NCBI): 28989
• RRID: AB_2142020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL11A, also known as NTMT1 (Nα-trimethyllysine hydrolase), is an enzyme that belongs to the methyltransferase-like (METTL) protein family. It plays a crucial role in epigenetic processes, particularly in the methylation of proteins, which can influence gene expression and other cellular functions. METTL11A is regulated by other members of the METTL family, such as METTL11B and METTL13, which can either activate or inhibit its function.