Tested Applications
| Positive WB detected in | A549 cells, Raji cells, HeLa cells, NCCIT cells, PC-3 cells, LNCaP cells |
| Positive IP detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26158-1-AP targets METTL14 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey, chicken, hamster, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14325 Product name: Recombinant human METTL14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-456 aa of BC007449 Sequence: LKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDELIAKSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKCWTWDDIMKLEIDEIAAPRSFIFLWCGSGEGLDLGRVCLRKWGYRRCEDICWIKTNKNNPGKTKTLDPKAVFQRTKEHCLMGIKGTVKRSTDGDFIHANVDIDLIITEEPEIGNIEKPVEIFHIIEHFCLGRRRLHLFGRDSTIRPGWLTVGPTLTNSNYNAETYASYFSAPNSYLTGCTEEIERLRPKSPPPKSKSDRGGGAPRGGGRGGTSAGRGRERNRSNFRGERGGFRGGRGGAHRGGFPPR Predict reactive species |
| Full Name | methyltransferase like 14 |
| Calculated Molecular Weight | 456 aa, 52 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC007449 |
| Gene Symbol | METTL14 |
| Gene ID (NCBI) | 57721 |
| RRID | AB_2800447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HCE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for METTL14 antibody 26158-1-AP | Download protocol |
| WB protocol for METTL14 antibody 26158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The METTL14 Polyclonal antibody (catalog number 26158-1-AP) has accumulated 227 citations. These appear across high-impact journals including Nature Communications, Cell Research, Molecular Cell, Cell Reports, Cell Death & Disease, Advanced Science, Cancer Research, and the Journal of Hematology & Oncology. Research fields span m6A RNA modification, cancer biology (tumorigenesis, metastasis, chemoresistance), ferroptosis, viral infections, diabetes, cardiovascular disease, neurology, and immunology, with applications predominantly in Western blot, RIP, IHC, and IF.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma
| ||
Mol Cancer The METTL3-YTHDC1 axis mediates architectural RNA m(6)A modification to modulate the integrity of chromatin TADs in MLLr + AML genome. | ||
Cell Metab Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11 | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Bioact Mater Macrophage membrane-coated nanovesicles for dual-targeted drug delivery to inhibit tumor and induce macrophage polarization |
Reviews
What customers say
All three reviews for this product are 5-star ratings, exclusively for Western Blot applications at a 1:1000 dilution. Users consistently report excellent band quality and strong performance. Two reviewers tested the antibody on HEK-293 cells and described the product as "highly recommended." Another user noted the bands were "excellent" and that the antibody contributed positively to their project. Overall, customer feedback is uniformly positive, highlighting reliable specificity and ease of use in WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (12-25-2025) | Antibodies are very good, bands are excellent, helped us in our project!
|
RASHMI (Verified Customer) (07-26-2024) | used this product, highly recommended
|
RASHMI (Verified Customer) (07-26-2024) | Used this product, highly recommended
|
