METTL2 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis of HeLa using 84257-1-RR (same clone as 84257-1-PBS)

  WB result of METTL2 antibody (84257-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL2 transfected HeLa cells. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

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  WB analysis using 84257-1-RR (same clone as 84257-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84257-1-RR (same clone as 84257-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01163-3

  Cytometric bead array standard curve of MP01163-3, METTL2B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84257-1-PBS. Detection antibody: 84257-3-PBS. Standard: Ag10410. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84257-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

• Featured Product
• KD/KO Validated

METTL2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 84257-1-PBS

Clone No.241490A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

METTL2B, 241490A3, EC:2.1.1.-, methyltransferase like 2B, Methyltransferase-like protein 2B

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Product Information

84257-1-PBS targets METTL2 as part of a matched antibody pair:

MP01163-3: 84257-1-PBS capture and 84257-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10410

  Product name: Recombinant human METTL2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-313 aa of BC107586

  Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS

• Full Name: methyltransferase like 2B
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 36~43 kDa
• GenBank Accession Number: BC107586
• Gene Symbol: METTL2B
• Gene ID (NCBI): 55798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6P1Q9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.