- Featured Product
- KD/KO Validated
METTL3 Recombinant antibody
METTL3 Recombinant Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2I9
Cat no : 80323-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells, HSC-T6 cells |
Positive IHC detected in | mouse testis tissue, human colon cancer tissue, human oesophagus cancer tissue, human lung cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
80323-1-RR targets METTL3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | METTL3 fusion protein Ag7110 |
Full Name | methyltransferase like 3 |
Calculated molecular weight | 64 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC001650 |
Gene symbol | METTL3 |
Gene ID (NCBI) | 56339 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for METTL3 antibody 80323-1-RR | Download protocol |
IHC protocol for METTL3 antibody 80323-1-RR | Download protocol |
IF protocol for METTL3 antibody 80323-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |