METTL5 Polyclonal antibody

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  WB analysis of K-562 using 16791-1-AP

  WB result of METTL5 antibody (16791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected K-562 cells.

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  WB analysis using 16791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16791-1-AP

  WB result of METTL5 antibody (16791-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells.

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  WB analysis of HeLa using 16791-1-AP

  WB result of METTL5 antibody (16791-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells.

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  IP experiment of PC-3 using 16791-1-AP

  IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug.

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  IHC staining of mouse kidney using 16791-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16791-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16791-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16791-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 16791-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

METTL5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16791-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: rat kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• RIP: See 1 publications below

Product Information

16791-1-AP targets METTL5 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10232

  Product name: Recombinant human METTL5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-205 aa of BC000921

  Sequence: MKKVRLKELESRLQQVDGFEKPKLLLEQYPTRPHIAACMLYTIHNTYDDIENKVVADLGCGCGVLSIGTAMLGAGLCVGFDIDEDALEIFNRNAEEFELTNIDMVQCDVCLLSNRMSKSFDTVIMNPPFGTKNNKGTDMAFLKTALEMARTAVYSLHKSSTREHVQKKAAEWKIKIDIIAELRYDLPASYKFHKKKSVDIEVDLI

• Full Name: methyltransferase like 5
• Calculated Molecular Weight: 209 aa, 24 kDa
• Observed Molecular Weight: 24-27 kDa
• GenBank Accession Number: BC000921
• Gene Symbol: METTL5
• Gene ID (NCBI): 29081
• RRID: AB_2142051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	EMBO Rep; The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.; Authors - Ming Xing ; KD Validated
human	WB	J Biol Chem; The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.; Authors - Caraline Sepich-Poore ; KO Validated
	WB	Front Cardiovasc Med; Loss of m6A Methyltransferase METTL5 Promotes Cardiac Hypertrophy Through Epitranscriptomic Control of SUZ12 Expression.; Authors - Yanchuang Han
human	WB,IHC	Bioengineered; The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.; Authors - Xiaojuan Liu ; KD Validated
human	RIP	Bioengineered; Long noncoding RNA UBA6-AS1 inhibits the malignancy of ovarian cancer cells via suppressing the decay of UBA6 mRNA.; Authors - Yaogang Wang
human	WB	Cell Rep; Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.; Authors - Bowen Rong ; KO Validated