Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16791-1-AP targets METTL5 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10232 Product name: Recombinant human METTL5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-205 aa of BC000921 Sequence: MKKVRLKELESRLQQVDGFEKPKLLLEQYPTRPHIAACMLYTIHNTYDDIENKVVADLGCGCGVLSIGTAMLGAGLCVGFDIDEDALEIFNRNAEEFELTNIDMVQCDVCLLSNRMSKSFDTVIMNPPFGTKNNKGTDMAFLKTALEMARTAVYSLHKSSTREHVQKKAAEWKIKIDIIAELRYDLPASYKFHKKKSVDIEVDLI Predict reactive species |
| Full Name | methyltransferase like 5 |
| Calculated Molecular Weight | 209 aa, 24 kDa |
| Observed Molecular Weight | 24-27 kDa |
| GenBank Accession Number | BC000921 |
| Gene Symbol | METTL5 |
| Gene ID (NCBI) | 29081 |
| RRID | AB_2142051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL5 antibody 16791-1-AP | Download protocol |
| IP protocol for METTL5 antibody 16791-1-AP | Download protocol |
| WB protocol for METTL5 antibody 16791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The METTL5 Polyclonal antibody (Cat# 16791-1-AP) has 27 citations. These appear in journals including Nature Metabolism, Advanced Science, Cell Death & Disease, Molecular Therapy, Oncogene, Cell Chemical Biology, Free Radical Biology & Medicine, Cell Reports, Fundamental Research, JCI Insight, EMBO Reports, Scientific Reports, iScience, FASEB Journal, Journal of Biological Chemistry, Cancer Medicine, Experimental Cell Research, Frontiers in Oncology, Future Oncology, Frontiers in Cardiovascular Medicine, World Journal of Gastrointestinal Oncology, Journal of Oral Pathology & Medicine, Bioengineered, and Journal of Thoracic Disease. Research fields span m6A RNA modification, cancer biology, ferroptosis, metabolism, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Nat Metab N6-methyladenosine (m6A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation
| ||
Adv Sci (Weinh) Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell-Induced Ferroptosis in Ovarian Cancer
| ||
Cell Death Dis METTL5 regulates SEPHS2-mediated selenoprotein synthesis to promote multiple myeloma survival and progression
| ||
Mol Ther METTL5 promotes fatty acid metabolism by modulating peroxisome to induce hepatocellular carcinoma recurrence after thermal ablation.
| ||
Cell Chem Biol N6-methyladenosine modification in 18S rRNA promotes tumorigenesis and chemoresistance via HSF4b/HSP90B1/mutant p53 axis |
