Home
Products
Primary Antibodies
METTL5 Polyclonal antibody, PBS Only (16791-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of K-562 using 16791-1-AP (same clone as 16791-1-PBS)
WB result of METTL5 antibody (16791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected K-562 cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
WB analysis using 16791-1-AP (same clone as 16791-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)
WB result of METTL5 antibody (16791-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)
WB result of METTL5 antibody (16791-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
IP experiment of PC-3 using 16791-1-AP (same clone as 16791-1-PBS)
IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 16791-1-AP (same clone as 16791-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

METTL5 Polyclonal antibody, PBS Only

Name: METTL5 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16791-1-PBS

METTL5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16791-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

16791-1-PBS targets METTL5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10232 Product name: Recombinant human METTL5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-205 aa of BC000921 Sequence: MKKVRLKELESRLQQVDGFEKPKLLLEQYPTRPHIAACMLYTIHNTYDDIENKVVADLGCGCGVLSIGTAMLGAGLCVGFDIDEDALEIFNRNAEEFELTNIDMVQCDVCLLSNRMSKSFDTVIMNPPFGTKNNKGTDMAFLKTALEMARTAVYSLHKSSTREHVQKKAAEWKIKIDIIAELRYDLPASYKFHKKKSVDIEVDLI Predict reactive species
Full Name	methyltransferase like 5
Calculated Molecular Weight	209 aa, 24 kDa
Observed Molecular Weight	24-27 kDa
GenBank Accession Number	BC000921
Gene Symbol	METTL5
Gene ID (NCBI)	29081
RRID	AB_2142051
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NRN9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.