METTL7A Polyclonal antibody

• Featured Product
• KD/KO Validated

METTL7A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17092-1-AP

Synonyms

AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

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Validation Data Gallery

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  WB analysis using 17092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17092-1-AP

  WB result of METTL7A antibody (17092-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

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  IHC staining of human liver cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 17092-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, mouse lung tissue, rat lung tissue, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17092-1-AP targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL7A fusion protein Ag10013
• Full Name: methyltransferase like 7A
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC008180
• Gene Symbol: METTL7A
• Gene ID (NCBI): 25840
• RRID: AB_2878348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.